[proofplan]
Set $A = -\Delta_D$, so $A$ is nonnegative and [self-adjoint](/page/Self-Adjoint%20Operators) on $L^2(\Omega)$. The spectral theorem gives a projection-valued spectral measure for $A$ and realizes the semigroup as the spectral functional calculus multiplier $e^{-t\lambda}$. Holomorphicity follows by applying the same functional calculus to $e^{-z\lambda}$ for $\operatorname{Re} z > 0$, while the derivative estimate follows from the scalar bound $\lambda e^{-t\lambda} \leq (et)^{-1}$ on $[0,\infty)$.
[/proofplan]
[step:Represent the semigroup by the spectral functional calculus]
Let $H := L^2(\Omega)$ with respect to $\mathcal{L}^n$, and let $A := -\Delta_D$ with domain $D(A) \subset H$. Let $\mathcal{L}(H)$ denote the [Banach space](/page/Banach%20Space) of bounded linear maps $T: H \to H$, equipped with the operator norm $\|T\|_{\mathcal{L}(H)}$. By hypothesis, $A$ is a nonnegative [self-adjoint](/page/Self-Adjoint%20Operators) operator on $H$.
We use the spectral theorem for nonnegative self-adjoint operators and its spectral functional calculus, results not yet represented by wiki theorem entries. Thus there exists a projection-valued measure $E_A$ on the Borel subsets of $[0,\infty)$ such that, for every bounded Borel function $m: [0,\infty) \to \mathbb{C}$, the operator $m(A) \in \mathcal{L}(H)$ is defined by the spectral functional calculus and satisfies
\begin{align*}
\|m(A)\|_{\mathcal{L}(H)} \leq \sup_{\lambda \in [0,\infty)} |m(\lambda)|.
\end{align*}
For $t \geq 0$, define the bounded Borel function $m_t: [0,\infty) \to \mathbb{C}$ by $m_t(\lambda) = e^{-t\lambda}$. Since $\Delta_D = -A$ generates the semigroup $(S(t))_{t \geq 0}$, the spectral functional calculus gives
\begin{align*}
S(t) = e^{-tA} = m_t(A).
\end{align*}
[/step]
[step:Extend the semigroup holomorphically to the right half-plane]
Let $\mathbb{C}_+ := \{z \in \mathbb{C} : \operatorname{Re} z > 0\}$. For each $z \in \mathbb{C}_+$, define the bounded Borel function $m_z: [0,\infty) \to \mathbb{C}$ by $m_z(\lambda)=e^{-z\lambda}$.
Since $\operatorname{Re} z > 0$ and $\lambda \geq 0$, one has
\begin{align*}
|m_z(\lambda)| = e^{-(\operatorname{Re} z)\lambda} \leq 1.
\end{align*}
Therefore $m_z(A) \in \mathcal{L}(H)$ and
\begin{align*}
\|m_z(A)\|_{\mathcal{L}(H)} \leq 1.
\end{align*}
Define $S: \mathbb{C}_+ \to \mathcal{L}(H)$ by $S(z)=m_z(A)$.
We prove that this map is holomorphic in the operator norm. Fix $z_0 \in \mathbb{C}_+$, and choose $r > 0$ such that $r < \operatorname{Re} z_0$. If $h \in \mathbb{C}$ satisfies $0 < |h| < r/2$, then $z_0 + h \in \mathbb{C}_+$ and the line segment from $z_0$ to $z_0+h$ lies in the half-plane $\{z \in \mathbb{C} : \operatorname{Re} z \geq \operatorname{Re} z_0 - r/2\}$.
Define $b_{z_0}: [0,\infty) \to \mathbb{C}$ by $b_{z_0}(\lambda) = \lambda e^{-z_0\lambda}$. Since $\operatorname{Re} z_0 > 0$, the function $b_{z_0}$ is bounded on $[0,\infty)$; indeed its absolute value is bounded by $(e\operatorname{Re} z_0)^{-1}$. Hence $A S(z_0)=b_{z_0}(A)$ is a bounded operator on $H$.
Define the difference-quotient multiplier $d_h: [0,\infty) \to \mathbb{C}$ by
\begin{align*}
d_h(\lambda) = \frac{e^{-(z_0+h)\lambda} - e^{-z_0\lambda}}{h} + \lambda e^{-z_0\lambda}.
\end{align*}
The spectral multiplier estimate gives
\begin{align*}
\left\|\frac{S(z_0+h)-S(z_0)}{h} + A S(z_0)\right\|_{\mathcal{L}(H)} \leq \sup_{\lambda \in [0,\infty)} |d_h(\lambda)|.
\end{align*}
We show that the supremum tends to $0$ as $h \to 0$. For each fixed $\lambda \geq 0$, the one-variable [holomorphic function](/page/Holomorphic%20Function) $\zeta \mapsto e^{-\zeta\lambda}$ gives $d_h(\lambda) \to 0$. To make this convergence uniform in $\lambda$, use Taylor's formula with integral remainder along the segment from $z_0$ to $z_0+h$:
\begin{align*}
e^{-(z_0+h)\lambda} = e^{-z_0\lambda} - h\lambda e^{-z_0\lambda} + h^2 \lambda^2 \int_0^1 (1-s)e^{-(z_0+sh)\lambda}\,d\mathcal L^1(s).
\end{align*}
Hence
\begin{align*}
|d_h(\lambda)| \leq |h|\lambda^2 e^{-(\operatorname{Re} z_0-r/2)\lambda}\int_0^1 (1-s)\,d\mathcal L^1(s).
\end{align*}
Since $\int_0^1 (1-s)\,d\mathcal L^1(s)=1/2$, and since the function $\lambda \mapsto \lambda^2 e^{-\beta\lambda}$ on $[0,\infty)$ has maximum $4e^{-2}\beta^{-2}$ when $\beta:=\operatorname{Re} z_0-r/2>0$, we obtain
\begin{align*}
\sup_{\lambda \in [0,\infty)} |d_h(\lambda)| \leq 2e^{-2}\beta^{-2}|h|.
\end{align*}
Therefore
\begin{align*}
\lim_{h\to 0}\left\|\frac{S(z_0+h)-S(z_0)}{h} + A S(z_0)\right\|_{\mathcal{L}(H)} = 0.
\end{align*}
Thus $S: \mathbb{C}_+ \to \mathcal{L}(H)$ is complex differentiable in operator norm at the arbitrary point $z_0$, with derivative $S'(z_0)=-A S(z_0)$. Therefore $S$ is a bounded holomorphic extension of the heat semigroup to $\mathbb{C}_+$.
[guided]
The goal is to extend the real-time formula $S(t) = e^{-tA}$ from $t > 0$ to complex time $z$ with positive real part. The spectral calculus reduces this operator-norm question to scalar estimates for the functions $\lambda \mapsto e^{-z\lambda}$ on the spectrum of $A$, which is contained in $[0,\infty)$ because $A$ is nonnegative.
Let
\begin{align*}
\mathbb{C}_+ := \{z \in \mathbb{C} : \operatorname{Re} z > 0\}.
\end{align*}
For each $z \in \mathbb{C}_+$, define $m_z: [0,\infty) \to \mathbb{C}$ by $m_z(\lambda)=e^{-z\lambda}$.
This is the correct scalar multiplier because $S(t)$ is $e^{-tA}$ for real $t > 0$. The condition $\operatorname{Re} z > 0$ is exactly what keeps this multiplier bounded on the nonnegative spectrum:
\begin{align*}
|m_z(\lambda)| = |e^{-z\lambda}| = e^{-(\operatorname{Re} z)\lambda} \leq 1
\end{align*}
for every $\lambda \geq 0$. The spectral calculus therefore defines a bounded operator $m_z(A) \in \mathcal{L}(H)$, and its norm satisfies
\begin{align*}
\|m_z(A)\|_{\mathcal{L}(H)} \leq \sup_{\lambda \in [0,\infty)} |m_z(\lambda)| \leq 1.
\end{align*}
We set $S: \mathbb{C}_+ \to \mathcal{L}(H)$ by $S(z)=m_z(A)$.
It remains to prove holomorphicity in the operator norm, not merely in scalar matrix coefficients. Fix $z_0 \in \mathbb{C}_+$, and choose $r > 0$ with $r < \operatorname{Re} z_0$. If $h \in \mathbb{C}$ and $0 < |h| < r/2$, then $z_0+h \in \mathbb{C}_+$, and every point $z_0 + sh$ with $s \in [0,1]$ satisfies
\begin{align*}
\operatorname{Re}(z_0+sh) \geq \operatorname{Re} z_0-r/2.
\end{align*}
Define $b_{z_0}: [0,\infty) \to \mathbb{C}$ by $b_{z_0}(\lambda)=\lambda e^{-z_0\lambda}$. Since $\operatorname{Re} z_0 > 0$, the scalar function $b_{z_0}$ is bounded on $[0,\infty)$, with
\begin{align*}
|b_{z_0}(\lambda)| \leq \lambda e^{-(\operatorname{Re} z_0)\lambda} \leq (e\operatorname{Re} z_0)^{-1}.
\end{align*}
Thus the spectral calculus defines $A S(z_0)=b_{z_0}(A)$ as a bounded operator on $H$.
To compute the derivative, define the difference-quotient error multiplier $d_h: [0,\infty) \to \mathbb{C}$ by
\begin{align*}
d_h(\lambda)=\frac{e^{-(z_0+h)\lambda}-e^{-z_0\lambda}}{h}+\lambda e^{-z_0\lambda}.
\end{align*}
The spectral multiplier estimate gives
\begin{align*}
\left\|\frac{S(z_0+h)-S(z_0)}{h}+A S(z_0)\right\|_{\mathcal{L}(H)} \leq \sup_{\lambda \in [0,\infty)} |d_h(\lambda)|.
\end{align*}
Therefore the operator-norm derivative will follow once this scalar supremum tends to $0$.
We use Taylor's formula with integral remainder for the one-variable holomorphic function $\zeta \mapsto e^{-\zeta\lambda}$ along the segment $\zeta = z_0+sh$, where $s \in [0,1]$. For each $\lambda \geq 0$,
\begin{align*}
e^{-(z_0+h)\lambda} = e^{-z_0\lambda}-h\lambda e^{-z_0\lambda}+h^2\lambda^2\int_0^1 (1-s)e^{-(z_0+sh)\lambda}\,d\mathcal{L}^1(s).
\end{align*}
Substituting this identity into the definition of $d_h$ gives
\begin{align*}
|d_h(\lambda)| \leq |h|\lambda^2 e^{-(\operatorname{Re} z_0-r/2)\lambda}\int_0^1 (1-s)\,d\mathcal{L}^1(s).
\end{align*}
The one-dimensional integral equals $1/2$. If
\begin{align*}
\beta := \operatorname{Re} z_0-r/2,
\end{align*}
then $\beta > 0$, and the function $\lambda \mapsto \lambda^2 e^{-\beta\lambda}$ on $[0,\infty)$ has maximum $4e^{-2}\beta^{-2}$. Consequently,
\begin{align*}
\sup_{\lambda \in [0,\infty)} |d_h(\lambda)| \leq 2e^{-2}\beta^{-2}|h|.
\end{align*}
The right-hand side tends to $0$ as $h \to 0$, so
\begin{align*}
\lim_{h\to 0}\left\|\frac{S(z_0+h)-S(z_0)}{h}+A S(z_0)\right\|_{\mathcal{L}(H)} = 0.
\end{align*}
Thus $S$ is complex differentiable in operator norm at $z_0$, with derivative $S'(z_0)=-A S(z_0)$. Since $z_0$ was arbitrary, $S: \mathbb{C}_+ \to \mathcal{L}(H)$ is holomorphic and extends the real heat semigroup.
[/guided]
[/step]
[step:Estimate the generator applied to the semigroup by a scalar spectral bound]
Fix $t > 0$. Define the scalar function $q_t: [0,\infty) \to \mathbb{C}$ by $q_t(\lambda)=\lambda e^{-t\lambda}$.
The function $q_t$ is bounded. Indeed, for $\lambda > 0$ the derivative of $\lambda \mapsto \lambda e^{-t\lambda}$ is $(1-t\lambda)e^{-t\lambda}$, so its maximum on $[0,\infty)$ occurs at $\lambda = t^{-1}$ and equals $(et)^{-1}$. Hence
\begin{align*}
\sup_{\lambda \in [0,\infty)} \lambda e^{-t\lambda} = \frac{1}{e t}.
\end{align*}
The spectral theorem also identifies domains of functions of $A$: a vector $w \in H$ belongs to $D(A)$ exactly when $\lambda \mapsto \lambda$ is square-integrable with respect to the spectral measure associated with $w$. Since $q_t(\lambda)=\lambda e^{-t\lambda}$ is bounded on $[0,\infty)$, the functional calculus implies $e^{-tA}H \subset D(A)$ and $A e^{-tA}=q_t(A)$ as a bounded operator on $H$. Therefore
\begin{align*}
\|A S(t)\|_{\mathcal{L}(H)} = \|A e^{-tA}\|_{\mathcal{L}(H)} \leq \frac{1}{e t}.
\end{align*}
Consequently, for every $u_0 \in H$,
\begin{align*}
\|A S(t)u_0\|_{L^2(\Omega)} \leq \frac{1}{e t}\|u_0\|_{L^2(\Omega)}.
\end{align*}
Since $A = -\Delta_D$, this also gives $S(t)u_0 \in D(A) = D(\Delta_D)$ and
\begin{align*}
\|\Delta_D S(t)u_0\|_{L^2(\Omega)} = \|A S(t)u_0\|_{L^2(\Omega)} \leq \frac{1}{e t}\|u_0\|_{L^2(\Omega)}.
\end{align*}
This proves the claimed estimate with $C = e^{-1}$, and hence with any $C \geq e^{-1}$.
[/step]