[guided]The local formula for $\Phi_V$ reduces the differential to the derivatives of the ratios $g_j=f_j/f_0$. We must connect those derivatives with sections that vanish at $x$, because tangent-vector separation is stated using such sections.
Choose $s_0\in V$ with $s_0(x)\neq 0$ and extend it to a basis $s_0,\dots,s_r$. In the affine chart determined by $s_0$, the map $\Phi_V$ is represented by
\begin{align*}
z &\mapsto \bigl(g_1(z),\dots,g_r(z)\bigr),
\end{align*}
where
\begin{align*}
g_j:U&\to\mathbb{C},\\
z&\mapsto \frac{f_j(z)}{f_0(z)}.
\end{align*}
Therefore $d(\Phi_V)_x(\xi)=0$ precisely when
\begin{align*}
dg_{j,x}(\xi)=0
\end{align*}
for every $j=1,\dots,r$.
Now define
\begin{align*}
t_j := s_j-g_j(x)s_0 \in V.
\end{align*}
This section vanishes at $x$, because in the trivialisation its representative is
\begin{align*}
h_j(z)=f_j(z)-g_j(x)f_0(z),
\end{align*}
and
\begin{align*}
h_j(x)=f_j(x)-\frac{f_j(x)}{f_0(x)}f_0(x)=0.
\end{align*}
Since $g_j=f_j/f_0$, differentiating at $x$ gives
\begin{align*}
dg_{j,x}(\xi)
&=
d\left(\frac{f_j}{f_0}\right)_x(\xi) \\
&=
\frac{df_{j,x}(\xi)f_0(x)-f_j(x)df_{0,x}(\xi)}{f_0(x)^2} \\
&=
\frac{d(f_j-g_j(x)f_0)_x(\xi)}{f_0(x)} \\
&=
\frac{dh_{j,x}(\xi)}{f_0(x)}.
\end{align*}
Because $f_0(x)\neq 0$, this vanishes exactly when $dh_{j,x}(\xi)=0$.
Every section $s\in V$ with $s(x)=0$ can be written as a linear combination of the sections $t_1,\dots,t_r$. Indeed, writing $s=\sum_{j=0}^r a_js_j$, the condition $s(x)=0$ implies
\begin{align*}
a_0f_0(x)+\sum_{j=1}^r a_jf_j(x)=0,
\end{align*}
so
\begin{align*}
a_0=-\sum_{j=1}^r a_j\frac{f_j(x)}{f_0(x)}=-\sum_{j=1}^r a_jg_j(x),
\end{align*}
and hence
\begin{align*}
s=\sum_{j=1}^r a_j(s_j-g_j(x)s_0)=\sum_{j=1}^r a_jt_j.
\end{align*}
Therefore the derivatives of all vanishing sections in the direction $\xi$ vanish if and only if all $dg_{j,x}(\xi)$ vanish. This proves that $d(\Phi_V)_x$ is injective exactly when $V$ separates tangent vectors at $x$.[/guided]