[guided]The purpose of the comparison field is to isolate the effect of the curvature lower bound. Let $v := D_tJ(0) \in T_{\gamma(0)}M$. Since $J(0)=0$ and $J$ is transverse, we have $v \perp \dot{\gamma}(0)$. Choose the simply connected complete Riemannian manifold $(\widetilde{M},\widetilde{g})$ of constant sectional curvature $k$, choose a unit-speed geodesic
\begin{align*}
\widetilde{\gamma}:[0,\pi/\sqrt{k}) \to \widetilde{M},
\end{align*}
and choose $\widetilde{v} \in T_{\widetilde{\gamma}(0)}\widetilde{M}$ with $\widetilde{v} \perp \dot{\widetilde{\gamma}}(0)$ and $|\widetilde{v}|_{\widetilde{g}}=|v|_g$. For $s \in [0,\pi/\sqrt{k})$, let
\begin{align*}
\widetilde{P}_s:T_{\widetilde{\gamma}(0)}\widetilde{M} \to T_{\widetilde{\gamma}(s)}\widetilde{M}
\end{align*}
denote parallel transport along $\widetilde{\gamma}$ from time $0$ to time $s$, and define
\begin{align*}
\widetilde{J}:[0,\pi/\sqrt{k}) &\to T\widetilde{M} \\
s &\mapsto \operatorname{sn}_k(s)\,\widetilde{P}_s\widetilde{v}.
\end{align*}
Because $\widetilde{P}_s\widetilde{v}$ is parallel and remains orthogonal to $\dot{\widetilde{\gamma}}(s)$, the constant-curvature Jacobi equation reduces to $D_s^2\widetilde{J}+k\widetilde{J}=0$. Also $\operatorname{sn}_k(0)=0$ and $\operatorname{sn}_k'(0)=1$, so $\widetilde{J}(0)=0$ and $D_s\widetilde{J}(0)=\widetilde{v}$. Since parallel transport preserves length,
\begin{align*}
|\widetilde{J}(s)|_{\widetilde{g}}
=
\operatorname{sn}_k(s)|\widetilde{v}|_{\widetilde{g}}
=
|D_tJ(0)|_g\,\operatorname{sn}_k(s).
\end{align*}
Now fix $t' \in (0,t)$. We invoke the lower-curvature half of Rauch comparison on $[0,t']$. This theorem compares transverse Jacobi fields whose initial values vanish and whose initial speeds have the same length. Its curvature hypothesis is that the sectional curvatures of the first manifold along planes containing the geodesic velocity are bounded below by the sectional curvatures of the comparison manifold. Let $K_{\widetilde{g}}$ denote the sectional curvature function of $(\widetilde{M},\widetilde{g})$. Here the curvature condition is exactly
\begin{align*}
K_g(\sigma) \geq k = K_{\widetilde{g}}(\widetilde{\sigma})
\end{align*}
for every plane $\sigma \subset T_{\gamma(s)}M$ containing $\dot{\gamma}(s)$ and every plane $\widetilde{\sigma} \subset T_{\widetilde{\gamma}(s)}\widetilde{M}$ containing $\dot{\widetilde{\gamma}}(s)$, with $0<s<t'$. The theorem also requires that the comparison be made before the first conjugate point along $\gamma$, because conjugate points are precisely where the differential of the exponential map can become singular and the length-ratio comparison may fail. This is why we apply the theorem first on $[0,t']$, where $t'<t$.
Rauch comparison gives
\begin{align*}
|J(t')|_g \leq |\widetilde{J}(t')|_{\widetilde{g}}.
\end{align*}
Substituting the explicit model length at time $t'$ yields
\begin{align*}
|J(t')|_g
\leq
|D_tJ(0)|_g\,\operatorname{sn}_k(t').
\end{align*}
Finally, $s \mapsto |J(s)|_g$ is continuous because $J$ is a smooth Jacobi field, and $s \mapsto \operatorname{sn}_k(s)$ is continuous because it is smooth. Letting $t' \uparrow t$ gives
\begin{align*}
|J(t)|_g
\leq
|D_tJ(0)|_g\,\operatorname{sn}_k(t).
\end{align*}
This is the desired infinitesimal upper bound on transverse separation before conjugacy.[/guided]