[step:Write the Sylvester determinant with fixed $Y$-degrees]Set $m:=\deg_Y F$ and $n:=\deg_Y G$. Since $F$ and $G$ have positive degree in $Y$, we have $m,n\ge 1$. Write $F(X,Y)=\sum_{i=0}^{m} f_i(X)Y^i$ and $G(X,Y)=\sum_{j=0}^{n} g_j(X)Y^j$, where $f_i,g_j\in k[X]$, $f_m\ne 0$, and $g_n\ne 0$.
Let $S(X)$ denote the $(m+n)\times(m+n)$ Sylvester matrix whose first $n$ rows are the coefficient rows of
\begin{align*}
Y^{n-1}F,\ Y^{n-2}F,\ \dots,\ F
\end{align*}
in the ordered basis
\begin{align*}
Y^{m+n-1},Y^{m+n-2},\dots,Y,1,
\end{align*}
and whose last $m$ rows are the coefficient rows of
\begin{align*}
Y^{m-1}G,\ Y^{m-2}G,\ \dots,\ G
\end{align*}
in the same ordered basis. By definition of the $Y$-resultant via the Sylvester matrix,
\begin{align*}
\operatorname{Res}_Y(F,G)=\det S(X)\in k[X].
\end{align*}
For $a\in k$, write $S(a)$ for the matrix obtained by evaluating every entry of $S(X)$ at $X=a$. Then
\begin{align*}
\operatorname{Res}_Y(F,G)(a)=\det S(a),
\end{align*}
because determinant is a polynomial expression in the entries of the matrix.[/step]