[guided]The straight-line interpolation is simple at the level of particles: a particle starting at $x$ and ending at $y$ follows the path $t\mapsto (1-t)x+ty$. The only subtlety is that the theorem asks for an Eulerian velocity $v_t(z)$ depending only on the current position $z$, not on the hidden pair $(x,y)$. If several pairs $(x,y)$ arrive at the same point $z$ at the same time $t$, the correct Eulerian velocity is the conditional average of $y-x$ over those pairs.
By the existence theorem for optimal transport plans with lower semicontinuous costs, there exists $\pi\in\Pi(\mu_0,\mu_1)$ minimizing the quadratic cost. Its hypotheses apply because the cost
\begin{align*}
c:\mathbb R^n\times\mathbb R^n&\to[0,\infty)
\end{align*}
\begin{align*}
(x,y)&\mapsto |x-y|^2
\end{align*}
is continuous and hence lower semicontinuous, while $\mu_0,\mu_1\in\mathcal P_2(\mathbb R^n)$ imply finite admissible cost:
\begin{align*}
\int_{\mathbb R^n\times\mathbb R^n}|x-y|^2\,d(\mu_0\otimes\mu_1)(x,y)<\infty.
\end{align*}
Thus we choose $\pi\in\Pi(\mu_0,\mu_1)$ such that
\begin{align*}
\int_{\mathbb R^n\times\mathbb R^n}|x-y|^2\,d\pi(x,y)=W_2^2(\mu_0,\mu_1).
\end{align*}
The cited existence result is not yet resolved to a wiki theorem ID: Existence of optimal transport plans for lower semicontinuous costs.
For each $t\in[0,1]$, define
\begin{align*}
S_t:\mathbb R^n\times\mathbb R^n&\to\mathbb R^n
\end{align*}
\begin{align*}
(x,y)&\mapsto (1-t)x+ty,
\end{align*}
and set $\rho_t:=(S_t)_\#\pi$. This means that, for every Borel set $A\in\mathcal B(\mathbb R^n)$,
\begin{align*}
\rho_t(A)=\pi(S_t^{-1}(A)).
\end{align*}
The endpoint conditions follow from $S_0(x,y)=x$ and $S_1(x,y)=y$, together with the marginal conditions on $\pi$:
\begin{align*}
\rho_{t=0}=\mu_0,\qquad \rho_{t=1}=\mu_1.
\end{align*}
For every $t\in[0,1]$, the measure $\rho_t$ has finite second moment because
\begin{align*}
\int_{\mathbb R^n}|z|^2\,d\rho_t(z)=\int_{\mathbb R^n\times\mathbb R^n}|(1-t)x+ty|^2\,d\pi(x,y)
\end{align*}
and $|(1-t)x+ty|^2\le 2|x|^2+2|y|^2$. Since the marginals of $\pi$ are $\mu_0$ and $\mu_1$, this gives
\begin{align*}
\int_{\mathbb R^n}|z|^2\,d\rho_t(z)\le 2\int_{\mathbb R^n}|x|^2\,d\mu_0(x)+2\int_{\mathbb R^n}|y|^2\,d\mu_1(y)<\infty.
\end{align*}
Thus $\rho_t\in\mathcal P_2(\mathbb R^n)$, as required for admissibility.
To prove narrow continuity, let $f\in C_b(\mathbb R^n)$. Then
\begin{align*}
\int_{\mathbb R^n}f(z)\,d\rho_t(z)=\int_{\mathbb R^n\times\mathbb R^n}f((1-t)x+ty)\,d\pi(x,y).
\end{align*}
For fixed $(x,y)$, the integrand is continuous in $t$, and it is bounded by $\|f\|_\infty$. Dominated convergence with respect to the probability measure $\pi$ gives continuity of $t\mapsto\int f\,d\rho_t$, which is exactly narrow continuity.
Now define the map that records both time and current position:
\begin{align*}
T:(0,1)\times\mathbb R^n\times\mathbb R^n&\to(0,1)\times\mathbb R^n
\end{align*}
\begin{align*}
(t,x,y)&\mapsto (t,(1-t)x+ty).
\end{align*}
Let
\begin{align*}
\eta:=\mathcal L^1\!\restriction_{(0,1)}\otimes\pi.
\end{align*}
The pushforward $\sigma:=T_\#\eta$ is precisely the space-time measure $d\rho_t(z)\,d\mathcal L^1(t)$, because for every nonnegative Borel function $\Psi:(0,1)\times\mathbb R^n\to[0,\infty]$,
\begin{align*}
\int\Psi(t,z)\,d\sigma(t,z)=\int_0^1\int_{\mathbb R^n\times\mathbb R^n}\Psi(t,(1-t)x+ty)\,d\pi(x,y)\,d\mathcal L^1(t).
\end{align*}
By the definition of $\rho_t$, the last expression equals
\begin{align*}
\int_0^1\int_{\mathbb R^n}\Psi(t,z)\,d\rho_t(z)\,d\mathcal L^1(t).
\end{align*}
The disintegration theorem now supplies conditional probability measures $\eta_{t,z}$ over each fiber $T^{-1}(\{(t,z)\})$. We use them to average the microscopic velocity $y-x$. Define
\begin{align*}
v:(0,1)\times\mathbb R^n&\to\mathbb R^n
\end{align*}
\begin{align*}
(t,z)&\mapsto \int_{(0,1)\times\mathbb R^n\times\mathbb R^n}(y-x)\,d\eta_{t,z}(s,x,y)
\end{align*}
on a full $\sigma$-measure set, and define it arbitrarily outside that set. This is the barycentric projection of the particle velocities; the variable $s$ is the time coordinate in the fiber measure, and the fiber condition forces $s=t$ for $\eta_{t,z}$-a.e. triple.
The action estimate is exactly Jensen's inequality. Since $a\mapsto |a|^2$ is convex,
\begin{align*}
|v_t(z)|^2\le \int_{\mathbb R^n\times\mathbb R^n}|y-x|^2\,d\eta_{t,z}(t,x,y)
\end{align*}
for $\sigma$-a.e. $(t,z)$. Integrating with respect to $d\sigma(t,z)=d\rho_t(z)\,d\mathcal L^1(t)$ gives
\begin{align*}
\int_0^1\int_{\mathbb R^n}|v_t(z)|^2\,d\rho_t(z)\,d\mathcal L^1(t)\le \int_0^1\int_{\mathbb R^n\times\mathbb R^n}|y-x|^2\,d\pi(x,y)\,d\mathcal L^1(t).
\end{align*}
Since $\mathcal L^1((0,1))=1$ and $\pi$ is optimal, the right-hand side equals $W_2^2(\mu_0,\mu_1)$. Hence the constructed dynamic competitor has action at most the static optimal cost.[/guided]